False negative results in the Roche assay for HDL-cholesterol
نویسندگان
چکیده
منابع مشابه
Early historical milestones in HDL-cholesterol assay.
without special precautions to compensate for the instability of thiol compounds in blood. One method for avoiding this mistake is to deproteinize the plasma or serum specimen before transport. In this study, rapid deproteinization preserved the disulfides as free analytes for at least 7 days in storage at 220 °C. However, plasma tHcy measurement is an even more effective method for assuring ac...
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We evaluated four new commercial methods for HDL-cholesterol determination. The three completely homogeneous assays were an immunoseparation-based (IS) method from Wako, a polyethylene glycol-modified enzyme (PEG) method from Boehringer Mannheim, and a synthetic polymer-based (SP) method from Genzyme. The fourth method was a new heterogeneous method in which lipoproteins are removed using dextr...
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HDL consists of two major subfractions, HDL2 and HDL3. This paper describes a simple method for assaying HDL subspecies by combining a single precipitation with a direct high density lipoprotein-cholesterol (HDL-C) assay. A precipitation reagent (0.06 ml) containing 1,071 U/ml heparin, 500 mmol/l MnCl2) and 12 mg/ml dextran sulfate was added to a serum (0.3 ml). The sample was incubated and cen...
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To the Editor: We report here the observation of a falsely decreased HDL-cholesterol (HDL-C) result obtained with the Roche HDL-C Plus assay (polyethylene glycol-modified enzymes, sulfated -cyclodextrin, and dextran sulfate; cat no. 1930648) in an asymptomatic 78-year-old woman (1 ). The total cholesterol of the patient was 3.2 mmol/L, the triglycerides were 1.3 mmol/L, and the HDL-C was suppre...
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BACKGROUND Accurate diagnosis of Clostridium difficile infection (CDI) is paramount for patient management. The wrong diagnosis places patients at risk, delays treatment, and/ or contributes to transmission of infection in the healthcare setting. Although amplification of the toxin B gene by polymerase chain reaction (PCR) is a sensitive method for detecting toxigenic C. difficile, false negati...
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ژورنال
عنوان ژورنال: Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
سال: 2003
ISSN: 0004-5632,1758-1001
DOI: 10.1258/000456303322326533